Table of Contents
- 1 Why should you never allow an objective lens to touch a slide?
- 2 What is the rule for touching lenses?
- 3 Which part of the microscope should never be touched when 40x objective lens is in position?
- 4 How important are the set of objectives of the microscope?
- 5 Can the course adjustment knob damage the high power objective lens?
- 6 How to use the coarse focus on a microscope?
Why should you never allow an objective lens to touch a slide?
REMOVING THE SLIDE FROM UNDER HIGHER POWER OBJECTIVES MAY CAUSE THE SLIDE SURFACE TO HIT THE OBJECTIVE LENS SINCE THE LENS IN THESE OBJECTIVES WILL BE VERY CLOSE TO THE SLIDE. THIS COULD RESULT IN DAMAGE TO THE LENS. b. TO ACHIEVE THE HIGHEST POSSIBLE RESOLUTION WHEN VIEWING THROUGH THE HIGHEST POWER OBJECTIVES.
Why should microscope lenses not be touch?
Never touch the lenses with your fingers. Your body produces an oil that smudges the glass. This oil can even etch the glass if left on too long. Use only LENS PAPER to clean the glass.
Why it is not good to touch the objective lens when focusing the microscope?
The objective lens should never touch the slide! Since your microscope is parfocal, meaning once the specimen is focused under one objective, it will be in aproximate focus under other objectives, the specimen should still be visible and still be in approximate focus.
What is the rule for touching lenses?
Your microscope slide should be prepared with a coverslip over the sample to protect the objective lenses if they touch the slide. Do not touch the glass part of the lenses with your fingers. Use only special lens paper to clean the lenses. Always keep your microscope covered when not in use.
What is the role of the objective lens?
The “objective lens” is the first-stage lens to form an image using electrons exiting from the specimen. The objective lens is the most important lens in the imaging lens system because the performance of this lens determines the image quality (resolution, contrast, etc).
What are advantages to using a wet mount slide preparation?
A wet-mount slide is when the sample is placed on the slide with a drop of water and covered with a coverslip, which holds it in place through surface tension. Advantages – This type of slide preparation allows you to view microscopic living things without them drying out.
Which part of the microscope should never be touched when 40x objective lens is in position?
Never turn your nosepiece back to the 40x objective when using oil or you will get oil all over that objective. Immersion oil should never be put on any other objective than the 100x!
What should you never do when you are looking through the high objective lens of the microscope?
NEVER USE THE COARSE FOCUS KNOB ON HIGH POWER! The high power lens should be very close to your slide when in proper focus. If you turn the coarse adjustment knob while on high power, the objective could easily break your slide.
What is the importance of lenses in the microscope?
While the modern microscope has many parts, the most important pieces are its lenses. It is through the microscope’s lenses that the image of an object can be magnified and observed in detail.
How important are the set of objectives of the microscope?
Microscope objectives are perhaps the most important components of an optical microscope because they are responsible for primary image formation and play a central role in determining the quality of images that the microscope is capable of producing.
What are the advantages and disadvantages of using a wet mount?
Terms in this set (4)
- Wet-mount: A microscope slide specimen is suspended in a drop of liquid located between the slide and coverslip.
- Different Types of Wet-mounts: -Water from organism’s natural habitat.
- Advantages: -Quick preparation.
- Disadvantages: -Movement of specimens make it difficult to draw/photograph.
What is the disadvantage in preparing a wet mount?
Possible problems of making a wet mount. The cover glass floats and moves: This is due to too much water. Remove water with the help of a tissue paper. Under no circumstances should there be water droplets on top of the cover glass.
Can the course adjustment knob damage the high power objective lens?
True or False: never use the course adjustment knob when focusing with the high-power objective lens, because this could break the slide or damage the lens. Once the specimen is focused properly with the course adjustment knob, you only need to turn the find adjustment knob ____________ of a turn to get a clear sharp image.
How to use a microscope to observe a slide?
We can now start observing the slide. Now take a slide and place it on the stage. If your microscope has stage clips, then use the clips to hold down the slide. If you have a mechanical stage, then place the slide all the way into the slide holder. Center the slide.
How do I adjust the low power objective?
Using the coarse focus knob, raise the stage all the way up. The low power objective is short and it should not crash into the slide. Do not look through the eyepiece just yet, just check that the objective does not touch the slide.
How to use the coarse focus on a microscope?
Make sure that the object that you want to look at is right over the hole in the stage. Otherwise you will not be able to see anything. Using the coarse focus knob, raise the stage all the way up. The low power objective is short and it should not crash into the slide.